Journal: Oncotarget

Article Title: Radioiodinated indomethacin amide for molecular imaging of cyclooxygenase-2 expressing tumors

doi: 10.18632/oncotarget.15437

Figure Lengend Snippet: First fluorescent tracers suitable for visualization of COX-2 in vivo

Article Snippet: Immunostaining of COX-1 was performed with goat polyclonal anti-COX-1 antibody and immunostaining of COX-2 with rabbit polyclonal anti-COX-2 antibody (both 1:1000, abcam, Cambridge, UK).

Techniques: In Vivo

Journal: Oncotarget

Article Title: Radioiodinated indomethacin amide for molecular imaging of cyclooxygenase-2 expressing tumors

doi: 10.18632/oncotarget.15437

Figure Lengend Snippet: ( A ) SDS and western blot analysis of COX-1 and COX-2 expression in HUVEC, HEK hCOX-2 nat and HEK hCOX-2 del cells in dependency on PMA- and Tet-stimulation, respectively. GAPDH served as a loading control. ( B ) SDS gel electrophoresis of cell lysates obtained from HEK hCOX-2del cells incubated with [I-125]5 and [I-125]6 and visualized by phosphorimager ( left ); subsequent western blot analysis with COX-2 specific antibody. ( C ) Intracellular localization of [I-125]5 and [I-125]6 in HEK hCOX-2 del cells detected by microautoradiography followed by a standard H&E staining. Arrows indicate the tracer localization.

Article Snippet: Immunostaining of COX-1 was performed with goat polyclonal anti-COX-1 antibody and immunostaining of COX-2 with rabbit polyclonal anti-COX-2 antibody (both 1:1000, abcam, Cambridge, UK).

Techniques: Western Blot, Expressing, SDS-Gel, Electrophoresis, Incubation, Staining

Journal: Oncotarget

Article Title: Radioiodinated indomethacin amide for molecular imaging of cyclooxygenase-2 expressing tumors

doi: 10.18632/oncotarget.15437

Figure Lengend Snippet: ( A ) μPET/CT molecular imaging of COX-2 with [I-124]6 in HT29 (upper panel) and HCT-116 (lower panel) xenografted SCID mice at 4 h p.i. Arrows indicate tumor. ( B ) Uptake of [I-124]6 in HT29 and HCT-116 tumors (in kBq/g tissue) (upper panel, left); tumor to muscle uptake ratio of [I-124]6 in HT29 and HCT-116 tumor xenografted SCID mice (upper panel, reight) at 4 h p.i.; gamma counter analysis of [I-124]6 biodistribution in HT29-xenografted SCID mice at 4 h p.i. (in %ID/g tissue) ( n = 5). ( C ) Immunhistological analysis of COX-1 and COX-2 in HT29 and HCT-116 xenografts. ( D ) Corresponding SDS and Western Blot analysis of COX-1 and COX-2 expression in HT29 and HCT-116 xenografts. Data are mean ± SD from five independent experiments. In B, * P < 0.05 by two-tailed Student´s t-test .

Article Snippet: Immunostaining of COX-1 was performed with goat polyclonal anti-COX-1 antibody and immunostaining of COX-2 with rabbit polyclonal anti-COX-2 antibody (both 1:1000, abcam, Cambridge, UK).

Techniques: Imaging, Western Blot, Expressing, Two Tailed Test